NEET-UG Biology · Class 12
Competent hosts and methods of gene transfer — NEET & JEE Questions
Competent hosts and methods of gene transfer is a topic in Biotechnology: Principles and Processes, a Class 12 Biology chapter of the NEET-UG syllabus. TestBudy holds 12 published NEET-UG questions on Competent hosts and methods of gene transfer, each with the marking scheme of the real paper.
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Competent hosts and methods of gene transfer MCQs with solutions
10 of the 12 published questions on this topic, exactly as they appear in a test. Answers and worked solutions come with your result rather than on this page.
Why are bacterial cells treated to become competent before transformation?
- A. To destroy their chromosomes
- B. To improve uptake of recombinant DNA
- C. To stop all normal membrane transport and permanently arrest cellular activity
- D. To make them produce spores
What is the purpose of the heat-shock step after treating bacterial cells with calcium ions?
- A. To remove every plasmid molecule from the treated bacterial population
- B. To denature all bacterial proteins
- C. To promote recombinant DNA uptake
- D. To stop cell division permanently
A bacterial cell is described as competent when it is able to
- A. take up foreign DNA efficiently
- B. perform photosynthesis
- C. form gametes
- D. destroy every plasmid
Calcium chloride treatment followed by heat shock is commonly used to help bacterial cells
- A. take up plasmid DNA
- B. synthesize antibodies
- C. undergo meiosis
- D. form viral capsids
A plant cell is difficult to transform using ordinary chemical methods. Which method can directly shoot DNA-coated particles into the cell?
- A. Chromatography
- B. Dialysis
- C. Centrifugation
- D. Biolistics
A pathogen is modified so that its disease-causing genes are removed but its natural DNA-delivery ability is retained. What is its role?
- A. A restriction enzyme
- B. A DNA-delivery vector
- C. A source of ATP only
- D. A selectable nutrient supplied to identify transformed cells in culture
A researcher wants to deliver recombinant DNA directly into a plant cell using microscopic DNA-coated particles. Which method is most appropriate?
- A. Gene gun or biolistics
- B. Heat shock of the whole plant
- C. Tollens test
- D. Centrifugation only
Which vector-mediated method is widely used to transfer genes into plants?
- A. Agrobacterium-mediated transfer
- B. Bacteriophage infection of human red blood cells
- C. Simple boiling of seeds
- D. Antibody injection
Which gene-transfer method introduces DNA directly into the nucleus of an animal cell using a fine needle?
- A. Microinjection
- B. Heat shock
- C. Gel electrophoresis
- D. Fermentation
A plasmid carrying antibiotic resistance is introduced into bacteria. After recovery, why are cells plated on medium containing that antibiotic?
- A. To select cells that received and express the plasmid marker
- B. To kill only transformed cells
- C. To force every bacterium to mutate
- D. To remove the plasmid from cells
Common mistakes in Competent hosts and methods of gene transfer
Recorded against questions in this topic that are not shown above, so none of these points at an answer you can see on this page.
- Assumes successful DNA transfer makes host physiology irrelevant.
- Assumes all expression hosts process proteins identically.
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